EFFECT OF THE TYPE OF DILUENT ON THE QUALITY OF FREE-RANGE CHICKEN SPERMATOZOA (GALLUS DOMESTICUS) STORED AT A TEMPERATURE OF 5°C
Keywords:
Native Chicken, Diluent, Ringer Lactate, Ringer Dextrose, Spermatozoa QualityAbstract
The native chicken is one of Indonesia's local livestock genetic resources that has great potential to be developed in Indonesia. Genetic quality can be improved through artificial insemination programs, which are highly dependent on the quality of the semen used. This study aims to analyze the effect of different diluents on the quality of native chicken spermatozoa stored at 5°C. This study is an experimental design with the treatment of two types of diluents namely (1) Ringer lactate and (2) Ringer dextrose as well as 0 days, 1 day, 2 days, 3 days, 4 days and 5 days as replicates. The observed variables were motility, viability, and abnormality of spermatozoa. Data analysis used was T-test. The results showed that motility, viability and abnormality of spermatozoa in Ringer lactate diluent were very good (p<0.01) and could survive up to 5 days compared to Ringerdextrose. The quality of spermatozoa in lactated Ringer's diluent and Ringer's dextrose at 5 days storage were, respectively, spermatozoa motility of 43% and 8%; spermatozoa viability of 64% and 14% while the abnormality value was 18% and 67%. Ringer lactate diluent is better at maintaining the quality of village chicken spermatozoa stored at 5°C than Ringer dextrose.
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Agustian, M. F., Ihsan, M. N., & Isnaini, N. (2014). Pengaruh lama simpan semen dengan pengencer tris aminomethan kuning telur pada suhu ruang terhadap kualitas spermatozoa kambing boer. TERNAK TROPIKA Journal of Tropical Animal Production, 15(2), 1–6.
Arifiantini, R. I., Wresdiyati, T., & Retnani, E. F. (2006). Pengujian morfologi spermatozoa sapi Bali (Bos sondaicus) menggunakan pewarnaan “williams.” J. Indontrop. Anim. Agric, 31(2), 105–110.
Ax, R.L., Dally, M., Didion, B.A., Lenz, R.W., Love, C.C., Varner, D.D., Hafez, B., and Bellin, M. E. (2000). Semen evaluation. In: Hafez, E.S.E., Hafez, B. (Eds.). Reproduction in Farm Animals. 7th Ed. Lippincott Williams and Wilkins, Philadelphia.
BPS. (2023). Data Populasi Ayam Kampung: Badan Pusat Statistik, Jakarta.
Caballero, B., Trugo, L., & Finglas, P. (2003). Encyclopedia of food sciences and nutrition: Volumes 1-10. (Issue Ed. 2).
Costa, J., Braga, P. C., Rebelo, I., Oliveira, P. F., & Alves, M. G. (2023). Mitochondria quality control and male fertility. Biology, 12(6), 827.
Danang, D. R., Isnaini, N., & Trisunuwati, P. (2012). Pengaruh lama simpan semen terhadap kualitas spermatozoa ayam kampung dalam pengencer ringer’s pada suhu 4 C. TERNAK TROPIKA Journal of Tropical Animal Production, 13(1), 47–57.
Ehling, C., Rath, D., Struckmann, C., Frenzel, A., Schindler, L., and Nieman, H. (2012). Utilization of Flowcytometric Techniques to Assess Membrane Integrity, Acrosome Reaction and Mitochondrial Activity in Boar Spermatozoa during Storage at 17°C. Theriogenology, 78(3): 558.
Feyisa ShiferawGaroma, F. S., Park YoungHyun, P. Y., Kim YoungMin, K. Y., Lee BoRam, L. B., Jung KyungMin, J. K., Choi SeongBok, C. S., Cho ChangYeon, C. C., & Han JaeYong, H. J. (2018). Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds.
Hafez, B. and Hafez, E. S. . (2008). Reproduction in Farm Animals. 7th Ed. Blackwell Publishing. Australia.
Isnaeni, M., Faidiban, O. R., & Tethool, A. N. (2019). Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam): Concentration and Motility Spermatozoa of Ayam Kampung (Gallus domesticus) in Ringer Laktat Diluents Adding Red Fruit Oil (Pandanus conoideus Lam). Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 9(2), 44–49.
Iswati, I., Natsir, M. H., Ciptadi, G., & Susilawati, T. (2021). Pengaruh NaCl fisiologis dan ringer laktat terhadap kualitas spermatozoa pada suhu ruang dan fertilitas telur ayam buras. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 23(1), 33–42.
Iswati, N. I., & Susilawati, T. (2017). Fertilitas spermatozoa ayam buras dengan penambahan antioksidan glutathione dalam pengencer ringer’s selama simpan dingin. Jurnal Ilmu-Ilmu Peternakan, 27(1), 107–115.
Kudratullah, K., & Sudrajat, A. S. (2021). Motilitas, viabilitas, dan morfologi spermatozoa ayam bangkok dengan pengencer dextrose dan Nacl fisiologis 10% pada penyimpanan suhu 5``C dan 26C. Indonesian Journal of Applied Science and Technology, 2(1), 1–9.
Kusumawati, E. D., Woli, S. L., Krisnaningsih, A. T. N., Susanto, W. E., Rahadi, S., & Susilawati, T. (2018). Motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5 C menggunakan pengencer dan lama simpan yang berbeda. Jurnal Ilmu Dan Teknologi Peternakan Tropis, 5(3), 102–105.
Nataamijaya, A. . (2010). Karakteristik penampilan kualitatif dan kuantitatif ayam kampung di berbagai wilayah di Indonesia. Jurnal Ilmu Ternak, 10(1): 1-6.
Nugroho, A. P., & Saleh, D. M. (2016). Motilitas dan abnormalitas spermatozoa ayam kampung dengan pengencer ringer laktat-putih telur dan lama simpan pada suhu 5 C selama 48 Jam. Acta Veterinaria Indonesiana, 4(1), 35–41.
Putranto, H. D., Nurmeiliasari, N., & Harferry, K. T. (2020). Studi kualitas semen ayam Burgo. Buletin Peternakan Tropis, 1(1), 10–15.
Rodríguez, A. G., Rodríguez, J. Z., Barreto, A., Sanabria-Barrera, S., Iglesias, J., & Morales, L. (2023). Impact of acute high glucose on mitochondrial function in a model of endothelial cells: Role of PDGF-C. International Journal of Molecular Sciences, 24(5), 4394.
Sumbalová, Z., Rausová, Z., Kucharská, J., Šranko, P., Harbulák, P., Svitok, P., López-Lluch, G., & Gvozdjáková, A. (2024). Platelet Mitochondrial Function and Endogenous Coenzyme Q10 Levels Could Be Used as Markers of Mitochondrial Health in Infertile Men: A Pilot Study. International Journal of Molecular Sciences, 26(1), 268.
Suprijatna, E., Atmomarsono, U., & Kartasudjana, R. (2005). Ilmu dasar ternak unggas. Penebar Swadaya, Jakarta, 227.
Susilawati, T. (2011). Spermatologi. Universitas Brawijaya.UB Press, Malang.
Ulus, E., Kusumawati, E. D., & Krisnaningsih, A. T. N. (2019). Pengaruh pengencer dan lama simpan semen ayam kampung pada suhu ruang terhadap motilitas dan viabilitas spermatozoa. Jurnal Sains Peternakan, 7(1), 29–40.
Varasofiari, L. N., Setiatin, E. T., & Sutopo, S. (2013). Evaluasi kualitas semen segar sapi Jawa Brebes berdasarkan lama waktu penyimpanan. Animal Agriculture Journal, 2(1), 201–208.
Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Universitas Brawijaya Press.
Yulnawati, H., Maheswari, H., Boediono, A., & Rizal, M. (2009). Potensi reproduksi dan upaya pengembangbiakan kerbau Belang Tana Toraja. Seminar Dan Lokakarya Nasional Kerbau, 152–158.
Yumte, K., Wantouw, B., & de Queljoe, E. (2013). Perbedaan Motilitas Spermatozoa Sapi Janta (Frisian Holstein) Setelah Pemberian Cairan Kristaloid-ringer Laktat. EBiomedik, 1(1).
Agustian, M. F., Ihsan, M. N., & Isnaini, N. (2014). Pengaruh lama simpan semen dengan pengencer tris aminomethan kuning telur pada suhu ruang terhadap kualitas spermatozoa kambing boer. TERNAK TROPIKA Journal of Tropical Animal Production, 15(2), 1–6.
Arifiantini, R. I., Wresdiyati, T., & Retnani, E. F. (2006). Pengujian morfologi spermatozoa sapi Bali (Bos sondaicus) menggunakan pewarnaan “williams.” J. Indontrop. Anim. Agric, 31(2), 105–110.
Ax, R.L., Dally, M., Didion, B.A., Lenz, R.W., Love, C.C., Varner, D.D., Hafez, B., and Bellin, M. E. (2000). Semen evaluation. In: Hafez, E.S.E., Hafez, B. (Eds.). Reproduction in Farm Animals. 7th Ed. Lippincott Williams and Wilkins, Philadelphia.
BPS. (2023). Data Populasi Ayam Kampung: Badan Pusat Statistik, Jakarta.
Caballero, B., Trugo, L., & Finglas, P. (2003). Encyclopedia of food sciences and nutrition: Volumes 1-10. (Issue Ed. 2).
Costa, J., Braga, P. C., Rebelo, I., Oliveira, P. F., & Alves, M. G. (2023). Mitochondria quality control and male fertility. Biology, 12(6), 827.
Danang, D. R., Isnaini, N., & Trisunuwati, P. (2012). Pengaruh lama simpan semen terhadap kualitas spermatozoa ayam kampung dalam pengencer ringer’s pada suhu 4 C. TERNAK TROPIKA Journal of Tropical Animal Production, 13(1), 47–57.
Ehling, C., Rath, D., Struckmann, C., Frenzel, A., Schindler, L., and Nieman, H. (2012). Utilization of Flowcytometric Techniques to Assess Membrane Integrity, Acrosome Reaction and Mitochondrial Activity in Boar Spermatozoa during Storage at 17°C. Theriogenology, 78(3): 558.
Feyisa ShiferawGaroma, F. S., Park YoungHyun, P. Y., Kim YoungMin, K. Y., Lee BoRam, L. B., Jung KyungMin, J. K., Choi SeongBok, C. S., Cho ChangYeon, C. C., & Han JaeYong, H. J. (2018). Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds.
Hafez, B. and Hafez, E. S. . (2008). Reproduction in Farm Animals. 7th Ed. Blackwell Publishing. Australia.
Isnaeni, M., Faidiban, O. R., & Tethool, A. N. (2019). Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam): Concentration and Motility Spermatozoa of Ayam Kampung (Gallus domesticus) in Ringer Laktat Diluents Adding Red Fruit Oil (Pandanus conoideus Lam). Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 9(2), 44–49.
Iswati, I., Natsir, M. H., Ciptadi, G., & Susilawati, T. (2021). Pengaruh NaCl fisiologis dan ringer laktat terhadap kualitas spermatozoa pada suhu ruang dan fertilitas telur ayam buras. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 23(1), 33–42.
Iswati, N. I., & Susilawati, T. (2017). Fertilitas spermatozoa ayam buras dengan penambahan antioksidan glutathione dalam pengencer ringer’s selama simpan dingin. Jurnal Ilmu-Ilmu Peternakan, 27(1), 107–115.
Kudratullah, K., & Sudrajat, A. S. (2021). Motilitas, viabilitas, dan morfologi spermatozoa ayam bangkok dengan pengencer dextrose dan Nacl fisiologis 10% pada penyimpanan suhu 5``C dan 26C. Indonesian Journal of Applied Science and Technology, 2(1), 1–9.
Kusumawati, E. D., Woli, S. L., Krisnaningsih, A. T. N., Susanto, W. E., Rahadi, S., & Susilawati, T. (2018). Motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5 C menggunakan pengencer dan lama simpan yang berbeda. Jurnal Ilmu Dan Teknologi Peternakan Tropis, 5(3), 102–105.
Nataamijaya, A. . (2010). Karakteristik penampilan kualitatif dan kuantitatif ayam kampung di berbagai wilayah di Indonesia. Jurnal Ilmu Ternak, 10(1): 1-6.
Nugroho, A. P., & Saleh, D. M. (2016). Motilitas dan abnormalitas spermatozoa ayam kampung dengan pengencer ringer laktat-putih telur dan lama simpan pada suhu 5 C selama 48 Jam. Acta Veterinaria Indonesiana, 4(1), 35–41.
Putranto, H. D., Nurmeiliasari, N., & Harferry, K. T. (2020). Studi kualitas semen ayam Burgo. Buletin Peternakan Tropis, 1(1), 10–15.
Rodríguez, A. G., Rodríguez, J. Z., Barreto, A., Sanabria-Barrera, S., Iglesias, J., & Morales, L. (2023). Impact of acute high glucose on mitochondrial function in a model of endothelial cells: Role of PDGF-C. International Journal of Molecular Sciences, 24(5), 4394.
Sumbalová, Z., Rausová, Z., Kucharská, J., Šranko, P., Harbulák, P., Svitok, P., López-Lluch, G., & Gvozdjáková, A. (2024). Platelet Mitochondrial Function and Endogenous Coenzyme Q10 Levels Could Be Used as Markers of Mitochondrial Health in Infertile Men: A Pilot Study. International Journal of Molecular Sciences, 26(1), 268.
Suprijatna, E., Atmomarsono, U., & Kartasudjana, R. (2005). Ilmu dasar ternak unggas. Penebar Swadaya, Jakarta, 227.
Susilawati, T. (2011). Spermatologi. Universitas Brawijaya.UB Press, Malang.
Ulus, E., Kusumawati, E. D., & Krisnaningsih, A. T. N. (2019). Pengaruh pengencer dan lama simpan semen ayam kampung pada suhu ruang terhadap motilitas dan viabilitas spermatozoa. Jurnal Sains Peternakan, 7(1), 29–40.
Varasofiari, L. N., Setiatin, E. T., & Sutopo, S. (2013). Evaluasi kualitas semen segar sapi Jawa Brebes berdasarkan lama waktu penyimpanan. Animal Agriculture Journal, 2(1), 201–208.
Wahjuningsih, S. (2021). Fisiologi Reproduksi dan Inseminasi Buatan pada Unggas. Universitas Brawijaya Press.
Yulnawati, H., Maheswari, H., Boediono, A., & Rizal, M. (2009). Potensi reproduksi dan upaya pengembangbiakan kerbau Belang Tana Toraja. Seminar Dan Lokakarya Nasional Kerbau, 152–158.
Yumte, K., Wantouw, B., & de Queljoe, E. (2013). Perbedaan Motilitas Spermatozoa Sapi Janta (Frisian Holstein) Setelah Pemberian Cairan Kristaloid-ringer Laktat. EBiomedik, 1(1).
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